Journal:

Article Title: Functional Analysis of the Gene Cluster Involved in Production of the Bacteriocin Circularin A by Clostridium beijerinckii ATCC 25752

doi: 10.1128/AEM.69.10.5839-5848.2003

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: When used together, chloramphenicol and erythromycin were employed at 2.5 μg/ml each for Lactobacillus saké ATCC 15521 or at 10 μg/ml (chloramphenicol) and 2 μg/ml (erythromycin) for E. faecalis JH2-2. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Description a Reference or source b C. beijerinckii ATCC 25752 Circularin A producer NIZO E. faecalis JH2-2 Plasmid-free derivative of E. faecalis JH-2 20 L. lactis NZ9000 Plasmid-free derivative of L. lactis MG1363, pepN :: nisRK 30 Lactobacillus sake ATCC 15521 Bacteriocin indicator lab collection E. coli Top10 F′ mcrA ( mrr-hsdRMS-mcrBC ) φ80 lacZM15 lacX74 recA1 deoR araD139 ( ara-leu ) 7697 galU galK rpsL ( Str r ) endA1 nupG Invitrogen E. coli DH5α supE44 Δ lacU169 (φ80 lacZ ΔM 15 ) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 17 Plasmids pMG36c Cm r , pWV01-based cloning vector carrying the strong lactococcal promoter P 32 Laboratory collection pMG36e Em r , gene expression vector carrying P 32 57 pCR21 Amp r Km r Invitrogen pCRAE Amp r Km r This study pIL253 Em r , theta replicating cloning vector 49 pIL-E Em r , pIL253 derivative with cirE under control of P 32 This study pIL-P32 Em r , pIL253 derivative with P 32 promoter This study pMGAE1 Cm r , pMG36c derivative with cirABCDE under control of P 32 This study pCir Cm r , derivative of pMGAE1 overexpressing circularin A This study pCirΔA Cm r , pCir Δ cirA This study pCirΔB Cm r , pCir Δ cirB This study pCirΔC Cm r , pCir Δ cirC This study pCirΔD Cm r , pCir Δ cirD This study pCirΔE Cm r , pCir Δ cirE This study pCirΔAE Cm r , pCir Δ cirA Δ cirE This study pCirΔBE Cm r , pCir Δ cirB Δ cirE This study pCirΔCE Cm r , pCir Δ cirC Δ cirE This study pCirΔDE Cm r , pCir Δ cirD Δ cirE This study pCirΔACE Cm r , pCir Δ cirA Δ cirC Δ cirE This study pCirΔABCE Cm r , pCir Δ cirA Δ cirB Δ cirC Δ cirE This study pCirΔACDE Cm r , pCir Δ cirA Δ cirC Δ cirD Δ cirE This study pMG-E Em r , pMG36e derivative with cirE under control of P 32 This study Open in a separate window a Amp r , ampicillin resistance; Cm r , chloramphenicol resistance; Em r , erythromycin resistance; Km r , kanamycin resistance. b NIZO, NIZO Food Research Ede, The Netherlands; Invitrogen, Breda, The Netherlands.

Techniques: Plasmid Preparation, Clone Assay, Expressing

Journal:

Article Title: VanD-Type Vancomycin-Resistant Enterococcus faecium 10/96A

doi: 10.1128/AAC.47.1.7-18.2003

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: The MICs of glycopeptides were determined by the method of Steers et al., with 10 5 CFU per spot on BHI agar after 24 h of incubation at 37°C ( 52 ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant properties Reference or source Strains E. coli Top10 F − mcrA Δ( mrr-hsdRMS-mcrBC ) φ80 lacZ ΔM15 Δ lacX74 deoR recA1 araD139 Δ( ara-leu ) 7697 galU galK rpsL endA1 nupG Invitrogen E. faecalis JH2-2 Fus r Rif r 32 E. faecium 10/96A Vm r Te r (VanD type) 23 BM4416 (N97-330) Vm r Te r (VanD type) 17, 43 BM4339 Vm r Te r (VanD type) 45 BM4409 BM4339/pAT662 ( P 2 ddlcat ) containing ddl gene of BM4147 21 BM4512 BM4339/pAT640 ( P 2 ddl G184S cat ) containing ddl gene of 10/96A This study Plasmids pCR-Blunt Km r , Zeocin r , oriR from colE1 , lacZ α, ccdB Invitrogen pAT79 oriR from pAMβ1, oriR from pUC, oriT from RK2, Sp r lacZ α P 2 cat 11 pAT637 632-bp PCR fragment ( vanD ′ vanX D ) of 10/96A cloned into pCR-Blunt This study pAT638 1,060-bp PCR fragment ( vanS D ′ vanY D ′) of 10/96A cloned into pCR-Blunt This study pAT639 3,725-bp PCR fragment ( vanR D S D ′ ORFA ORFB vanS D ′ vanY D ′) of 10/96A cloned into pCR-Blunt This study pAT640 1,135-bp Sac I- Xba I PCR fragment ( ddl G184S ) of 10/96A cloned into pAT79 This study pAT635 672-bp Sac I- Xba I PCR fragment ( vanX D ) of BM4339 cloned into pAT79 This study pAT633 1,153-bp Sac I- Xba I PCR fragment ( vanY D ) of BM4339 cloned into pAT79 This study pAT636 672-bp Sac I- Xba I PCR fragment ( vanX D ) of 10/96A cloned into pAT79 This study pAT634 1,153-bp Sac I- Xba I PCR fragment ( vanY D ) of 10/96A cloned into pAT79 This study Open in a separate window Strains and plasmids used in this study

Techniques: Plasmid Preparation, Clone Assay

Journal:

Article Title: Functional Analysis of the Gene Cluster Involved in Production of the Bacteriocin Circularin A by Clostridium beijerinckii ATCC 25752

doi: 10.1128/AEM.69.10.5839-5848.2003

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: When used together, chloramphenicol and erythromycin were employed at 2.5 μg/ml each for Lactobacillus saké ATCC 15521 or at 10 μg/ml (chloramphenicol) and 2 μg/ml (erythromycin) for E. faecalis JH2-2. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Description a Reference or source b C. beijerinckii ATCC 25752 Circularin A producer NIZO E. faecalis JH2-2 Plasmid-free derivative of E. faecalis JH-2 20 L. lactis NZ9000 Plasmid-free derivative of L. lactis MG1363, pepN :: nisRK 30 Lactobacillus sake ATCC 15521 Bacteriocin indicator lab collection E. coli Top10 F′ mcrA ( mrr-hsdRMS-mcrBC ) φ80 lacZM15 lacX74 recA1 deoR araD139 ( ara-leu ) 7697 galU galK rpsL ( Str r ) endA1 nupG Invitrogen E. coli DH5α supE44 Δ lacU169 (φ80 lacZ ΔM 15 ) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 17 Plasmids pMG36c Cm r , pWV01-based cloning vector carrying the strong lactococcal promoter P 32 Laboratory collection pMG36e Em r , gene expression vector carrying P 32 57 pCR21 Amp r Km r Invitrogen pCRAE Amp r Km r This study pIL253 Em r , theta replicating cloning vector 49 pIL-E Em r , pIL253 derivative with cirE under control of P 32 This study pIL-P32 Em r , pIL253 derivative with P 32 promoter This study pMGAE1 Cm r , pMG36c derivative with cirABCDE under control of P 32 This study pCir Cm r , derivative of pMGAE1 overexpressing circularin A This study pCirΔA Cm r , pCir Δ cirA This study pCirΔB Cm r , pCir Δ cirB This study pCirΔC Cm r , pCir Δ cirC This study pCirΔD Cm r , pCir Δ cirD This study pCirΔE Cm r , pCir Δ cirE This study pCirΔAE Cm r , pCir Δ cirA Δ cirE This study pCirΔBE Cm r , pCir Δ cirB Δ cirE This study pCirΔCE Cm r , pCir Δ cirC Δ cirE This study pCirΔDE Cm r , pCir Δ cirD Δ cirE This study pCirΔACE Cm r , pCir Δ cirA Δ cirC Δ cirE This study pCirΔABCE Cm r , pCir Δ cirA Δ cirB Δ cirC Δ cirE This study pCirΔACDE Cm r , pCir Δ cirA Δ cirC Δ cirD Δ cirE This study pMG-E Em r , pMG36e derivative with cirE under control of P 32 This study Open in a separate window a Amp r , ampicillin resistance; Cm r , chloramphenicol resistance; Em r , erythromycin resistance; Km r , kanamycin resistance. b NIZO, NIZO Food Research Ede, The Netherlands; Invitrogen, Breda, The Netherlands.

Techniques: Plasmid Preparation, Clone Assay, Expressing

Journal: Applied and Environmental Microbiology

Article Title: Green Fluorescent Protein-Labeled Monitoring Tool To Quantify Conjugative Plasmid Transfer between Gram-Positive and Gram-Negative Bacteria

doi: 10.1128/AEM.05578-11

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Then, the λ phage t 0 terminator was cloned downstream of the oriT region via XbaI and XhoI sites with primer pair t 0 -term- Xba I-fw and t 0 -term- Xba I-re using the expression vector pQTEV ( 35 ) as the template, thus generating plasmid pVA- gfp - oriT ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Bacterial strain or plasmid Relevant genotype or phenotype a Source or reference Bacterial strains Escherichia coli XL10 Δ( mcrA ) 183 Δ( mcrCB-hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F′ proAB lacI q Z ΔM15 Tn 10 (Tet r ) Amy Cm r ] Stratagene Enterococcus faecalis JH2-2 Rif r Fus r 23 OG1RF Rif r Fus r 10 OG1X Sm r 22 T9 Tet r 21 Bacillus subtilis subsp. natto DSM 4451 Sm r DSMZ b Plasmids pIP501 Tra Cm r MLS r 12 pMSP3535VA pVA380-1 and ColE1 replicons nisRK P nisA Km r 6 pJP rel GFP P rel gfp 31 pQTEV P t4 lacI q His 7 Amp r 35 pJP rel GFP- oriT pJP rel GFP oriT pIP501 This work pVA- gfp - oriT pMSP3535VA gfp oriT pIP501 t 0 -term pQTEV This work Open in a separate window a MLS r , macrolide-lincosamide-streptogramin B resistance; Hte, high transformation efficiency; Amy, amylase. b DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen (German Collection of Microorganisms and Cell Cultures).

Techniques: Plasmid Preparation